Functional Differences in the Multiple Hemocyanins of the Horseshoe Crab, Limulus polyphemus L
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چکیده
منابع مشابه
Functional differences in the multiple hemocyanins of the horseshoe crab, Limulus polyphemus L.
Hemocyanin in the hemolymph of the horseshoe crab, Limulus polyphemus L., is a high-molecular-weight copper protein which binds oxygen cooperatively and shows a higher oxygen affinity at pH 7 than at pH 9. Treatment with EDTA (ethylenediaminetetra-acetate) disaggregates the hemocyanin molecules and abolishes both the reverse Bohr effect and cooperative oxygen binding. Chloride ions interact wit...
متن کاملUsing the Horseshoe Crab, Limulus Polyphemus, in Vision Research
The American horseshoe crab, Limulus Polyphemus is one of the oldest creatures on earth, and the animal continues to play an indispensable role in biomedical research. Not only does their blood contain special cells that scientists use to detect bacteriotoxins in our medicines, but their eyes also contain a neural network that has provided much insight about physiological processes operating in...
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The fine structure of the cardiac muscle of the horseshoe crab, Limulus polyphemus, has been studied with respect to the organization of its contractile material, and the structure of its organelles and the cell junctions. Longitudinal sections show long sarcomeres (5.37 micro at L(max)), wide A bands (2.7 micro), irregular Z lines, no M line, and no apparent H zone. Transverse sections through...
متن کاملDevelopmental ecology of the American horseshoe crab Limulus polyphemus
During spawning events, horseshoe crab eggs are released from the female’s oviducts, and fertilized by one or more males. Eggs are shaped by the female into discrete clutches deposited in nests at depths of 10−20 cm on intertidal estuarine beaches. Distinguishing between fresh eggs and the early developmental stages is obfuscated by the large amount of dense, opaque yolk. The first unambiguous ...
متن کاملBlood Collection from the American Horseshoe Crab, Limulus Polyphemus
The horseshoe crab has the best-characterized immune system of any long-lived invertebrate. The study of immunity in horseshoe crabs has been facilitated by the ease in collecting large volumes of blood and from the simplicity of the blood. Horseshoe crabs show only a single cell type in the general circulation, the granular amebocyte. The plasma has the salt content of sea water and only three...
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ژورنال
عنوان ژورنال: Proceedings of the National Academy of Sciences
سال: 1974
ISSN: 0027-8424,1091-6490
DOI: 10.1073/pnas.71.6.2558